期刊
JOURNAL OF IMMUNOLOGY
卷 179, 期 7, 页码 4563-4570出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.179.7.4563
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- NINDS NIH HHS [NS014435, R01 NS041435] Funding Source: Medline
Previous studies have shown that central memory T (T-CM) cells predominantly use the calcium-dependent potassium channel KCa3.1 during acute activation, whereas effector memory T (T-EM) cells use the voltage-gated potassium channel Kv1.3. Because Kv1.3-specific pharmacological blockade selectively inhibited anti-CD3-mediated proliferation, whereas naive T cells and T-CM cells escaped inhibition due to up-regulation of KCa3.1, this difference indicated a potential for selective targeting of the T,m population. We examined the effects of pharmacological Kv1.3 blockers and a dominant-negative Kv1.x construct on T cell subsets to assess the specific effects of Kv1.3 blockade. Our studies indicated both T-CM and T-EM CD4(+) T cells stimulated with anti-CD3 were inhibited by charybdotoxin, which can block both KCa3.1 and Kv1.3, whereas margatoxin and Stichodactyla helianthus toxin, which are more selective Kv1.3 inhibitors, inhibited proliferation and IFN-gamma production only in the T-EM subset. The addition of anti-CD28 enhanced proliferation of freshly isolated cells and rendered them refractory to S. helianthus, whereas chronically activated T-EM cell lines appeared to be costimulation independent because Kv1.3 blockers effectively inhibited proliferation and IFN-gamma regardless of second signal. Transduction of CD4+ T cells with dominant-negative Kv1.x led to a higher expression of CCR7(+) T-EM phenotype and a corresponding depletion of T-EM. These data provide further support for Kv1.3 as a selective target of chronically activated T-EM without compromising naive or T-CM immune functions. Specific Kv1.3 blockers may be beneficial in autoimmune diseases such as multiple sclerosis in which T-EM are found in the target organ.
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