期刊
BIOMATERIALS
卷 28, 期 29, 页码 4231-4239出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2007.06.014
关键词
Ti; cytokines; monocytes; endotoxin; inflammation and wound healing
资金
- NIDDK NIH HHS [DK054932, R01 DK054932, R56 DK054932, R01 DK054932-09] Funding Source: Medline
This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78 +/- 0.12 mu m) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 mu g/mL lipopolysaccha ride (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1 beta, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-alpha, IL-6, MIP-1 alpha, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles. (c) 2007 Elsevier Ltd. All rights reserved.
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