期刊
JOURNAL OF NEUROCHEMISTRY
卷 103, 期 2, 页码 784-792出版社
WILEY
DOI: 10.1111/j.1471-4159.2007.04816.x
关键词
avian; circadian; photoreceptor; retina; voltage-gated calcium channel
资金
- NEI NIH HHS [R01EY017452, R01 EY017452-01A1, R01 EY017452] Funding Source: Medline
Photoreceptors are non-spiking neurons, and their synapses mediate the continuous release of neurotransmitters under the control of L-type voltage-gated calcium channels (VGCCs). Photoreceptors express endogenous circadian oscillators that play important roles in regulating photoreceptor physiology and function. Here, we report that the L-type VGCCs in chick cone photoreceptors are under circadian control. The L-type VGCC currents are greater when measured during the subjective night than during the subjective day. Using antibodies against the VGCC alpha 1C and VGCC alpha 1D subunits, we found that the immunofluorescence intensities of both VGCC alpha 1C and VGCC alpha 1 D in photoreceptors are higher during the subjective night. However, the mRNA levels of VGCC alpha 1D, but not VGCC alpha 1C, are rhythmic. Nocturnal increases in L-type VGCCs are blocked by manumycin A, PD98059, and KN93, which suggest that the circadian output pathway includes Ras, Erk, and calcium-calmodulin dependent kinase II. In summary, four independent lines of evidence show that the L-VGCCs in cone photoreceptors are under circadian-control.
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