期刊
GENESIS
卷 45, 期 10, 页码 618-624出版社
WILEY-LISS
DOI: 10.1002/dvg.20334
关键词
cleft palate; glomerulus; kidney; odd-skipped; Osr2; palate development; Cre; loxP
资金
- NIDCR NIH HHS [DE013681, R01 DE013681-08, R01 DE013681-06, R01 DE013681, R01 DE013681-07] Funding Source: Medline
Mammalian palate development is a multistep process, involving initial bilateral downward outgrowth of the palatal shelves from the oral side of the maxillary processes, followed by stage-specific palatal shelf elevation to the horizontal position above the developing tongue and subsequent fusion of the bilateral palatal shelves at the midline to form the intact roof of the oral cavity. While mutations in many genes have been associated with cleft palate pathogenesis, the mo lecular mechanisms regulating palatal shelf growth, patterning, and elevation are not well understood. Genetic studies of the molecular mechanisms controlling palate development in mutant mouse models are often complicated by early embryonic lethality or gross craniofacial malformation. We report here the development of a mouse strain for tissue-specific analysis of gene function in palate development. We inserted an IresCre bicistronic expression cassette into the 31 untranslated region of the mouse Osr2 gene through gene targeting. We show, upon crossing to the R26R reporter mice, that Cre expression from the Osr2(Irescre) knockin allele activated P-galactosidase expression specifically throughout the developing palatal mesenchyme from the onset of palatal shelf outgrowth. In addition, the Osr2(Irescre) mice display exclusive. Cre-mediated recombination in the glomeruli tissues derived from the metanephric mesenchyme and complete absence of Cre activity in other epithelial and mesenchymal tissues in the developing metanephric kidney. These data indicate that the Osr2(Irescre) knockin mice provide a unique tool for tissue specific studies of the molecular mechanisms regulating palate and kidney development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据