Speed-mapping quantitative trait loci using microarrays

We developed a rapid, economical method for high-resolution quantitative trait locus ( QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F-2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms ( SFPs) to map previously identified and additional QTLs affecting lifespan.