期刊
CHROMATOGRAPHIA
卷 66, 期 7-8, 页码 571-575出版社
VIEWEG
DOI: 10.1365/s10337-007-0354-z
关键词
gas chromatography; extraction pine seeds; Pinus pinaster; cis-verbenol; verbenone
Results from a systematic study of the factors affecting extraction of cis-verbenol and verbenone from pine seeds are presented. Five extraction conditions were investigated: extraction solvent, method of extraction, extraction temperature, volume of solvent, and the ratio of the mass of sample to the amount of extraction solvent. The resulting optimized method uses magnetic-stirring-assisted extraction of pine seeds (5 g) with ethyl acetate (75 ml.) for 20 min, at room temperature. RSDs were less than 5% for both compounds. GC-FID was used for quantification of cis-verbenol and verbenone in the extracts.
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