期刊
TRENDS IN GENETICS
卷 23, 期 10, 页码 478-480出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tig.2007.08.007
关键词
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Making multiple sequence alignments is one of the more commonplace procedures in modern biology. Multiple alignments are typically generated by feeding sequences into the alignment program from the N-terminus to the C-terminus. Recent results show that if the same sequences are processed from the C- to the N-terminus, a different alignment is often obtained. Because phylogenetic trees are built from alignments, the resulting trees can also differ. The new findings highlight sequence alignment as a crucial step in molecular evolutionary studies and provide straightforward measures to assess alignment reliability.
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