期刊
JOURNAL OF VIROLOGY
卷 81, 期 20, 页码 11461-11467出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02423-06
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资金
- Intramural NIH HHS Funding Source: Medline
- NIAID NIH HHS [R01 AI054952, AI-054952, R21 AI054952] Funding Source: Medline
Cytotoxic T lymphocytes (CTLs) are important for the control of virus replication during respiratory infections. For human metapneumovirus (hMPV), an H-2(d) -restricted CTL epitope in the M2-2 protein has been described. In this study, we screened the hMPV F, G, N, M, M2-1, and M2-2 proteins using three independent algorithms to predict H-2(d) CTL epitopes in BALB/c mice. A dominant epitope (GYIDDNQSI) in positions 81 to 89 of the antitermination factor M2-1 and a subdominant epitope (SPKAGLLSL) in N307-315 were detected during the anti-hMPV CTL response. Passive transfer of CD8(+) T-cell lines against M2-1(81-89) and N307-315 protected Ragl(-/-) mice against hMPV challenge. Interestingly, diversification of CTL targets to include multiple epitopes was observed after repetitive infections. A subdominant response against the previously described M2-2 epitope was detected after the third infection. An understanding of the CTL response against hMPV is important for developing preventive and therapeutic strategies against the virus.
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