期刊
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 75, 期 2, 页码 91-96出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jri.2007.03.017
关键词
human; placenta; amnion; pluripotent stem cells; immunofluorescence
The placenta contains different populations of stem/progenitor cells such as mesenchymal, hematopoietic, trophoblastic and pluripotent stem cells. Although some tissue-specific stem cells are restricted to particular parts of the placenta, the localization of embryonic stem cell-like cells in term human placenta has not been determined. We have used immunofluorescence staining techniques with antibodies to pluripotent stem cell antigens, SSEA-3, SSEA-4, TRA 1-60 and TRA 1-81, and confocal microscopic analysis to identify and localize stem cells within the placenta. Stem cell marker-positive cells were found in amnion but not in choriodecidua, tissues known to contain hermatopoietic and trophoblastic stem cells. Amniotic mesenchymal cells did not react with these pluripotent stem cell markers, while all amniotic epithelial cells reacted with at least one antibody. The TRA 1-60 and TRA 1-81 positive cells were solitary and present throughout the surface of amniotic membrane without a specific pattern of distribution, whereas SSEA-3 was negative and SSEA-4 was weakly positive on all amniotic epithelial cells. These data suggest that the human amnion contains stem cell-like cells at different states of differentiation. Human term amnion may be useful source of pluripotent stem cells for regenerative medicine. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据