期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 8, 期 10, 页码 1039-1051出版社
MDPI AG
DOI: 10.3390/i8101039
关键词
rUBISCO; red algae; crystallization; phase transition.
We have isolated ribulose-1,5-bisphosphate-carboxylase/ oxygenase ( RUBISCO) from the red algae Galdieria Sulphuraria. The protein crystallized in two different crystal forms, the I422 crystal form being obtained from high salt and the P21 crystal form being obtained from lower concentration of salt and PEG. We report here the crystallization, preliminary stages of structure determination and the detection of the structural phase transition in the P2(1) crystal form of G. sulphuraria RUBISCO. This red algae enzyme belongs to the hexadecameric class ( L8S8) with an approximate molecular weight 0.6MDa. The phase transition in G. sulphuraria RUBISCO leads from two hexadecamers to a single hexadecamer per asymmetric unit. The preservation of diffraction power in a phase transition for such a large macromolecule is rare.
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