期刊
FERTILITY AND STERILITY
卷 88, 期 -, 页码 1150-1157出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2007.01.036
关键词
hamster embryo; lighting; preimplantation development; Hsp70; reactive oxygen species (ROS); apoptosis
资金
- National Research Foundation of Korea [2006-07531] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Objective: To optimize embryo culture system by evaluating lighting effect during in vitro manipulation. Design: Randomized prospective study using an animal model. Setting: Gamete and Stem Cell Biotechnology Laboratory at Seoul National University in Korea. Animal(s): Twelve- to 15-week-old hamsters (Mesocricetus auratus). Intervention(S): Two-cell embryos were manipulated in vitro under lighting of different intensities and wavelengths. Main Outcome Measure(s): Preimplantation development, Hsp70 expression, reactive oxygen species (ROS) generation, and blastomere apoptosis. Result(s): A significant. increase in morula and blastocyst fort-nation was detected at 200 lux compared with 900 lux visible ray lighting, but not compared with 500 lux. At 200-lux lighting,red (620-750 nm) ray yielded the best development, whereas blue (445-500 nm) decreased blastocyst formation. Compared with the visible ray, Hsp70 expression and ROS generation in morula were increased in the blue ray but decreased in the red ray lighting. The blue ray also reduced blastocyst quality with increasing blastomere apoptosis. Conclusion(s): Specific wavelength of visible ray increases Hsp70 expression, ROS generation, and blastomere apoptosis. Therefore, removing this stress factor improves embryo development. (Fertil Steril (R) 2007;88(Suppl 2): 1150-7. (C)2007 by American Society for Reproductive Medicine.)
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