Influence of two-phase system composition on biocatalytic properties of β-galactosidase preparations

A comparison was made between the catalytic properties of beta-galactosidase from Penicillium canescens and Aspergillus oryzae using lactose in two-phase systems of an organic solvent and water. It was shown that the ability of beta-galactosidase preparations to synthesize galactooligosaccharides depends on the type of the solvent used (cyclohexane, hexane, toluene) and on its proportion in the two-phase system. The use of cyclohexane in the reaction medium stabilized the activity of beta-galactosidase from P. canescens which was immobilized on tosylated cotton cloth, and enabled its multiple use in galactooligosaccharides synthesis.