期刊
BIOMEDICAL MICRODEVICES
卷 9, 期 5, 页码 627-635出版社
SPRINGER
DOI: 10.1007/s10544-007-9051-9
关键词
microfluidics; concentration gradients; ladder chamber; cell migration; neutrophil chemotaxis
We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was successfully observed in soluble chemoattractant (IL-8) gradient. The Ladder Chamber's simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels.
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