期刊
NATURE METHODS
卷 4, 期 10, 页码 817-821出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1097
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资金
- NIGMS NIH HHS [R01 GM067193-04, R01 GM067193, GM 067193] Funding Source: Medline
Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.
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