期刊
JOURNAL OF MAGNETIC RESONANCE
卷 188, 期 2, 页码 301-310出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2007.07.004
关键词
spin-state selection; homonuclear decoupling; C-13 NMR; protonless NMR; protein assignment; ferritin; superoxide dismutase
Attempts are made to efficiently decouple C-13 nuclei without significant loss of coherence during the application of the decoupling package. Such attempts are based on the (SE)-E-3 spin-state selection method. A newly developed double (SE)-E-3 ((DSE)-E-3) is particularly efficient for C-alpha detection for proteins as large as 480 kDa. (C) 2007 Elsevier Inc. All rights reserved.
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