期刊
BIOMEDICAL MICRODEVICES
卷 9, 期 5, 页码 675-679出版社
SPRINGER
DOI: 10.1007/s10544-007-9076-0
关键词
centrifugal microfluidics; siphon; sedimentation; metering sample preparation; whole blood
In this paper, we present a novel and fully integrated centrifugal microfluidic lab-on-a-disk for rapid colorimetric assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t=150 s in passive, globally hydrophilized structures which obviate the need for intricate local hydrophobic surface patterning. Our technology features a plasma extraction structure (V=500 nL, C < 5%) where the purified plasma (c(RBC)< 0.11%) is centrifugally separated, metered by an overflow and subsequently extracted by a siphon-based principle through a hydrophilic extraction channel into the detection chamber.
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