期刊
BIOELECTROMAGNETICS
卷 28, 期 7, 页码 519-528出版社
WILEY-LISS
DOI: 10.1002/bem.20336
关键词
ELF-PEMF; bone cell; bioreactor; DNA synthesis; alkaline phosphatase
Bone tissue engineering is an interdisciplinary field involving both engineers and cell biologists, whose main purpose is to repair bone anatomical defects and maintain its functions. A novel system that integrates pulsed electromagnetic fields (PEMFs) and bioreactors was applied to bone tissue engineering for regulating osteoblast proliferation and differentiation in vitro. Osteoblasts were acquired from the calvaria of newborn Wistar rats and isolated after sequential digestion. Poly(DL-lactic-co-glycolic acid) (PLGA) scaffolds were made by the solvent merging/particulate leaching method. Osteoblasts were seeded into porous PLGA scaffolds with 85% porosity and cultured in bioreactors for the 18-day culture period. Cells were exposed to PEMF pulsed stimulation with average (rms) amplitudes of either 0. 13, 0.24, or 0.32 mT amplitude. The resulting induced electric field waveform consisted of single, narrow 300 Vs quasi-rectangular pulses with a repetition rate of 7.5 Hz. The results showed that PEMF stimulation for 2 and 8 h at 0. 13 mT increased the cell number on days 6 and 12, followed by a decrease on day 18 using 8 h stimulation. However, ALP activity was decreased and then increased on days 12 and 18, respectively. On the other hand, PEMF-treated groups (irrespective of the stimulation time) at 0.32 mT inhibited cell proliferation but enhanced ALP activity during the culture period. These findings suggested that PEMF stimulation with specific parameters had an effect on regulating the osteoblast proliferation and differentiation. This novel integrated system may have potential in bone tissue engineering.
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