期刊
FEBS LETTERS
卷 581, 期 24, 页码 4704-4710出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.08.066
关键词
photosynthesis; CP29; CP26; neoxanthin; light-harvesting complex; Photosystem II
The location of the neoxanthin binding site in CP26 and CP29 was investigated by site-directed mutagenesis. The crystallographic structure of LHCII shows that the binding of neoxanthin to the NI site is stabilised by an H bond with a tyrosine in the lumenal loop. This residue is conserved in CP26 and CP29. Mutation of this tyrosine into phenylalanine induced specific loss of neoxanthin without affecting violaxanthin binding. In contrast to previous proposals, it is thus concluded that also in these minor antenna complexes neoxanthin is accommodated in the NI site. The characteristics of this binding site in the different antenna complexes are discussed. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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