期刊
EMBO JOURNAL
卷 26, 期 19, 页码 4283-4291出版社
WILEY
DOI: 10.1038/sj.emboj.7601843
关键词
DNA polymerase gamma; electron microscopy; mitochondrial DNA; processivity; replication
资金
- NIBIB NIH HHS [P41 EB002181, 5 P41 EB2181] Funding Source: Medline
- NIGMS NIH HHS [R01 GM029681] Funding Source: Medline
We used electron microscopy to examine the structure of human DNA pol gamma, the heterotrimeric mtDNA replicase implicated in certain mitochondrial diseases and aging models. Separate analysis of negatively stained preparations of the catalytic subunit, pol gamma A, and of the holoenzyme including a dimeric accessory factor, pol gamma B-2, permitted unambiguous identification of the position of the accessory factor within the holoenzyme. The model explains protection of a partial chymotryptic cleavage site after residue L-549 of pol gamma A upon binding of the accessory subunit. This interaction region is near residue 467 of pol gamma A, where a disease-related mutation has been reported to impair binding of the B subunit. One pol gamma B subunit dominates contacts with the catalytic subunit, while the second B subunit is largely exposed to solvent. A model for pol c is discussed that considers the effects of known mutations in the accessory subunit and the interaction of the enzyme with DNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据