期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1167, 期 1, 页码 85-94出版社
ELSEVIER
DOI: 10.1016/j.chroma.2007.08.043
关键词
chemiluminescence detection; photochemical derivatization; HPLC; cerium (IV); rhodamine B; tetracyclines; water
A simple, selective and sensitive method has been developed for the simultaneous determination of tetracycline, oxytetracycline, chlorotetracycline, demeclocycline, doxycycline and meclocycline based on reversed-phase high-performance liquid chromatography with chemiluminescence detection. The procedure was based on the chemiluminescent enhancement by photoirradiated tetracyclines of the cerium (IV)-rhodamine B system in sulphuric acid medium. The six tetracyclines were separated on an Aquasil-C18 column with a gradient elution using a mixture of acetonitrile and 0.1 mol L-1 phosphate buffer as mobile phase, photoderivatized using a photoreactor consisting of a tube reactor coil of PFA and a 8W Xenon lamp. Under the optimized conditions, the method was validated with respect to linearity, precision, limits of detection and quantification and accuracy. The relative standard deviation (RSD) on intra-day precision was below 10% and detection limits ranged between 0.12 and 0.34 mu g L-1. The proposed method has been successfully applied to the determination of tetracyclines in surface water samples. A possible mechanism of the chemiluminescence in the system is discussed. (C) 2007 Elsevier B.V. All rights reserved.
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