期刊
CANCER LETTERS
卷 255, 期 2, 页码 263-274出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2007.04.012
关键词
mesothelin; CA125; biobody; tumor cell attachment; ovarian cancer; peritoneal metastasis
类别
资金
- NCI NIH HHS [P50 CA83636, P50 CA083636] Funding Source: Medline
Preventing peritoneal implantation of carcinoma cells could prolong ovarian cancer patient remission and survival. Peritoneal cells constitutively express mesothelin, a ligand for CA 125 that is expressed by tumor cells. Thus blocking CA 125/ mesothelin-dependent cell attachment may prevent or delay peritoneal metastatic recurrence. We developed a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. Using a novel yeast-expression system to produce secreted, in vivo biotinylated proteins and biobodies (Bbs), we generated anti-mesothelin Bbs. Anti-mesothelin Bbs derived from high affinity yeast-display scFv detected both membrane-bound and soluble mesothelins and inhibited CA125/mesothelin-dependent cell attachment. Published by Elsevier Ireland Ltd.
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