期刊
FEBS LETTERS
卷 581, 期 25, 页码 4917-4920出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.09.022
关键词
PCNA; cancer cell; normal cell; two-dimensional gel electrophoresis
In order to clarify the status of PCNA in normal and transformed cells, we performed analysis of this protein by 2D-PAGE, Western blot and mass spectrometry. All the cell lines examined contained the major PCNA form (pI 4.57/30 kDa), that is not post-translationally modified. In addition to the major form, two minor isoforms (pI 4.52/30 kDa and pI 4.62/30 kDa) were also detected in all the cell lines examined. However, the level of PCNA in cancer cells is 5-6 folds higher than those in primary and most of the immortalized cells. Taken together, the significant difference in PCNA status between cancer and normal cells is not at the post-translational modifications but in the overall levels of PCNA. Crown Copyright (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. All rights reserved.
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