期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 104, 期 42, 页码 16592-16597出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0708183104
关键词
F420 fluorescence; fluorimetry; ice cores; dissolved molecules; single cells
资金
- Office of Polar Programs (OPP)
- Directorate For Geosciences [0738658] Funding Source: National Science Foundation
- Office of Polar Programs (OPP)
- Directorate For Geosciences [944348] Funding Source: National Science Foundation
Two known habitats for microbial metabolism in ice are surfaces of mineral grains and liquid veins along three-grain boundaries. We propose a third, more general, habitat in which a microbe frozen in ice can metabolize by redox reactions with dissolved small molecules such as CO2, O-2, N-2, CO, and CH4 diffusing through the ice lattice. We show that there is an adequate supply of diffusing molecules throughout deep glacial ice to sustain metabolism for >10(5) yr. Using scanning fluorimetry to map proteins (a proxy for cells) and F420 (a proxy for methanogens) in ice cores, we find isolated spikes of fluorescence with intensity consistent with as few as one microbial cell in a volume of 0.16 mu l with the protein mapper and in 1.9 mu l with the methanogen mapper. With such precise localization, it should be possible to extract single cells for molecular identification.
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