期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 55, 期 21, 页码 8367-8371出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf0720584
关键词
angiotensin I-converting enzyme (ACE) inhibitor; peptide; column-switching; high-performance liquid chromatography (HPLC); casein hydrolysate
A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three anti hypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1 % trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 +/- 0.037 mg/g, 2.50 +/- 10.26 mg/g, and 0.096 +/- 0.008 mg/g, respectively.
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