期刊
EMBO JOURNAL
卷 26, 期 20, 页码 4335-4346出版社
WILEY
DOI: 10.1038/sj.emboj.7601861
关键词
copper; cytochrome c oxidase; heme a; mitochondria; Shy1
资金
- NIDDK NIH HHS [DK P30 072437] Funding Source: Medline
- NIEHS NIH HHS [R01 ES003817, ES 03817, R37 ES003817] Funding Source: Medline
The assembly of cytochrome c oxidase (CcO) in yeast mitochondria is shown to be dependent on a new assembly factor designated Coa1 that associates with the mitochondrial inner membrane. Translation of the mitochondrial-encoded subunits of CcO occurs normally in coa1 Delta cells, but these subunits fail to accumulate. The respiratory defect in coa1D cells is suppressed by highcopy MSS51, MDJ1 and COX10. Mss51 functions in Cox1 translation and elongation, whereas Cox10 participates in the biosynthesis of heme a, a key cofactor of CcO. Respiration in coa1D and shy1 Delta cells is enhanced when Mss51 and Cox10 are coexpressed. Shy1 has been implicated in formation of the heme a(3)-Cu-B site in Cox1. The interaction between Coa1 and Cox1, and the physical and genetic interactions between Coa1 and Mss51, Shy1 and Cox14 suggest that Coa1 coordinates the transition of newly synthesized Cox1 from the Mss51: Cox14 complex to the heme a cofactor insertion involving Shy1. coa1D cells also display a mitochondrial copper defect suggesting that Coa1 may have a direct link to copper metallation of CcO.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据