期刊
SCIENCE
卷 318, 期 5850, 页码 619-622出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1148735
关键词
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资金
- Engineering and Physical Sciences Research Council [EP/D060192/1] Funding Source: researchfish
- EPSRC [EP/D060192/1] Funding Source: UKRI
Carbohydrate recognition is biologically important but intrinsically challenging, for both nature and host-guest chemists. Saccharides are complex, subtly variable, and camouflaged by hydroxyl groups that hinder discrimination between substrate and water. We have developed a rational strategy for the biomimetic recognition of carbohydrates with all-equatorial stereochemistry (beta-glucose, analogs, and homologs) and have now applied it to disaccharides such as cellobiose. Our synthetic receptor showed good affinities, not unlike those of some lectins (carbohydrate-binding proteins). Binding was demonstrated by nuclear magnetic resonance, induced circular dichroism, fluorescence spectroscopy, and calorimetry, all methods giving self-consistent results. Selectivity for the target substrates was exceptional; minor changes to disaccharide structure (for instance, cellobiose to lactose) caused almost complete suppression of complex formation.
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