Description of Bursaphelenchus anamurius sp n. (Nematoda: Parasitaphelenchidae) from Pinus brutia in Turkey

Bursaphelenchus anamurius sp. n. isolated from wilted Turkish pine (Pinus brutia Ten.) wood is described. The new species shares several characters with the hofmanni-group sensu Brasch (2001). It is characterised by a body length of 633 (540-749) mu m and 744 (638-867) mu m for males and females, respectively, stout body (a = 29 in male and 27 in female), stylet 14 mu m long in both males and females, lateral field with three lines, length of postuterine branch one-third to one-half of the vulva to anus distance, conoid female tail (c' = 3.4) with a finely rounded or pointed terminus, very delicate and relatively straight spicules 10 (9-11) mu m long with compact capitulum, broad, not distinctly offset condylus and strong rostrum with a more or less rounded tip lacking a cucullus, shovel-shaped bursa and the presence of seven caudal papillae. Bursaphelenchus anamurius sp. n. differs from other species of the hofmanni-group mainly by having smaller, unusually shaped spicules, shorter condylus and no cucullus. The new species can be differentiated from other Bursaphelenchus species morphologically and by means of ITS-RFLP patterns, particularly by digestion of the PCR product with RsaI, HaeIII, MspI, HinfI and AluI.