期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 392, 期 -, 页码 73-79出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2015.09.010
关键词
Mass spectrometry; Desorption electrospray ionization; Desalting and enrichment; Online enzyme digestion; Phosphoprotein
资金
- NSF [CHE-1149367, CHE-1455554]
- NNSFC [21328502]
- National Institutes of Health [R01GM103479]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1455554] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1149367] Funding Source: National Science Foundation
Desorption electrospray ionization mass spectrometry (DESI-MS) is a recent and important advance in the field that has extensive applications in surface analysis of solid samples but has also been extended to analysis of liquid samples. The liquid sample DESI typically employs a piece of fused silica capillary to transfer liquid sample for ionization. In this study, we present the improvement of liquid sample DESI-MS by replacing the sample transfer silica capillary with a trap column filled with chromatographic stationary phase materials (e.g., C4, C18). This type of trap column/liquid sample DESI can be used for trace analysis of organics and biomolecules such as proteins/peptides (in nM concentration) in high salt content matrices. Furthermore, when the sample transfer capillary is modified with enzyme covalently bound on its inside capillary wall, fast digestion (<6 min) of proteins such as phosphoproteins can be achieved and the online digested proteins can be directly ionized using DESI with high sensitivity. The latter is ascribed to the freedom to select favorable spray solvent for the DESI analysis. Our data show that liquid sample DESI-MS with a modified sample transfer capillary has significantly expanded utility in bioanalysis. (C) 2015 Elsevier B.V. All rights reserved.
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