Disposable screen-printed carbon electrodes for dual Electrochemiluminescence/Amperometric detection: Sequential injection analysis of oxalate

This work describes the development and application of an electrochemical cell specifically designed for disposable screen printed carbon electrodes (SPCE) suitable for simultaneous electrochemiluminescence (ECL) and amperometric detection in sequential injection analysis. The flow system with facility for photomultiplier tube via a fiber optic facing the SPCE is user-friendly and makes the detection process very easy to operate. Instead of the need to constant deliver the chemiluminescence (CL) reagents to the reaction zone, sequential injection analysis allows a considerable reduction in the consumption of the sample and expensive CL reagents (such as Ru(bpy)(3)(2+) salts). The utility of the analyzer was demonstrated for the detection of oxalate based on the ECL reaction with Ru(bpy)(3)(2+). Under optimized conditions, in the presence of 100 mu M Ru(bpy)(3)(2+), the linear ranges of peak current and 3 3 ECL light intensity for oxalate distinctly varied from 10 mu M to 5 mM and 0.1 mu M to 100 mu W, respectively. In other words, the linear detection can be covered over a four-order range with the combination of these two signals.