期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 12, 期 6, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2812554
关键词
extended depth of focus; deconvolution; 3-D image acquisition; inverse problems; image formation theory; microscopy
资金
- NIBIB NIH HHS [R01 EB002344] Funding Source: Medline
- NIGMS NIH HHS [R01 GM49798, R01 GM55708] Funding Source: Medline
We derive a method for extended depth-of-focus imaging, i.e., a method to render a 2-D image of a thick specimen, such that all the structures within the specimen appear in focus and with greatly increased contrast. We acquire a single image while moving the specimen through focus. The resulting image, which is severely blurred and has very low contrast, is then deconvolved. In the deconvolved image, the entire depth of the specimen is in focus. Because the image is collected continuously while the specimen moves through focus, the acquisition time is short. Likewise, because the deconvolution is done in 2-D, it is done very quickly even with an iterative algorithm. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
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