期刊
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
卷 19, 期 3, 页码 133-137出版社
INFORMA HEALTHCARE
DOI: 10.3109/13506129.2012.700287
关键词
beta-Amyloid; A beta; Alzheimer's disease; Peptide synthesis; SPPS
资金
- Korea Institute of Science and Technology [2V02950, 2E22310]
Feasible and reproducible synthesis of full-length A beta peptides has been one of the major challenges in Alzheimer's disease research. By using dimethyl sulfoxide as an anti-aggregation solvent and as an agent to promote double-coupling of two phenylalanine that frequently experience residual deletion, we developed a reliable manual Fmoc solid phase peptide synthesis procedure to produce biologically active A beta in large quantities at relatively high purity. The amyloidogenic activity of the synthesized A beta was confirmed via thioflavin T assay, transmission electron microscopic analysis and electrophoresis.
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