Amphibacillus sediminis sp nov., an endospore-forming bacterium isolated from lake sediment in Japan

A facultatively anaerobic, moderately alkaliphilic, Gram-positive, spore-forming and rod-shaped bacterial strain, Shu-P-Ggiii25-2(T), was isolated from lake sediment in Shizuoka, Japan, and was characterized taxonomically using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that the novel isolate clustered with the type strain of Amphibacillus xylanus and it exhibited sequence similarities of 94.9-95.6% to the type strains of species of the genus Amphibacillus. Isoprenoid quinones and oxidase and catalase activities were not detected for strain Shu-P-Ggiii25-2(T). The DNA G + C content was 42.3 mol%, the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and the major cellular fatty acids were iso-C-14:0, iso-C-15:0, anteiso-C-15:0, C-16:0 and iso-C-16:0. On the basis of phylogenetic position and phenotypic properties, strain Shu-P-Ggiii25-2(T) represents a novel species of the genus Amphibacillus, for which the name Amphibacillus sediminis sp. nov. is proposed. The type strain is Shu-P-Ggiii25-2(T) (=MBIC08269(T)=IAM 15428(T) =KCTC 13120(T)). An emended description of the genus Amphibacillus is also given.