期刊
INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH
卷 77, 期 6, 页码 369-375出版社
VERLAG HANS HUBER
DOI: 10.1024/0300-9831.77.6.369
关键词
lycopene; geometrical isomers; UV-vis spectroscopy; mass spectrometry; NMR spectroscopy
Five geometrical isomers of lycopene were isolated from a photoisomerized mixture on a semi-preparative C-30 column and characterized by UV-vis spectroscopy, mass spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. H-1 NMR and 2D NMR measurements were used to unambiguously assign the double bond configuration of five isomers: (5Z,9'Z)-, (9Z)-, (5Z,9Z)-, (all-E)-, and (5Z)-lycopene. This will allow other laboratories to use the results for their C30 HPLC. In addition, it is possible to investigate structure-activity relationships for these lycopene isomers which will improve the understanding of their physiological role in biological tissues.
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