期刊
PLANT CELL REPORTS
卷 26, 期 11, 页码 1933-1939出版社
SPRINGER
DOI: 10.1007/s00299-007-0415-7
关键词
Arabidopsis thaliana; DNA fragmentation; in vitro; callogenesis; endoreduplication; polyploidization
Leaf explants of diploid (2n = 2x = 10) and autotetraploid (2n = 4x = 20) plants of Arabidopsis thaliana ecotype Columbia were cytologically and cytogenetically analysed to determine the time and the mechanisms of the process of polyploidization. The first polyploid cells were observed after the third day of culture in both genotypes of explants. Polyploid cells were the result of pre-existing mixoploidy in explants of A. thaliana. Other factors such as endoreduplication, endomitosis, abnormal microtubules arrangement and DNA damage may have induced polyploidization during early stages of callogenesis.
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