期刊
AMINO ACIDS
卷 46, 期 5, 页码 1353-1365出版社
SPRINGER WIEN
DOI: 10.1007/s00726-014-1700-8
关键词
Cysteine; Cysteine dioxygenase; Cystathionine gamma-lyase; Cystathionine beta-synthase; Hydrogen sulfide; Thiosulfate; Mice; Hepatocytes
资金
- National Institute of Diabetes and Digestive and Kidney Diseases [DK-056649]
- Charles University in Prague [PRVOUK-P24/LF1/3]
- project OPPK [CZ.2.16/3.1.00/24012]
- Ministry of Science and Higher Education (MNISW), Republic of Poland
- Grants-in-Aid for Scientific Research [26104509] Funding Source: KAKEN
The oxidation of cysteine in mammalian cells occurs by two routes: a highly regulated direct oxidation pathway in which the first step is catalyzed by cysteine dioxygenase (CDO) and by desulfhydration-oxidation pathways in which the sulfur is released in a reduced oxidation state. To assess the effect of a lack of CDO on production of hydrogen sulfide (H2S) and thiosulfate (an intermediate in the oxidation of H2S to sulfate) and to explore the roles of both cystathionine gamma-lyase (CTH) and cystathionine beta-synthase (CBS) in cysteine desulfhydration by liver, we investigated the metabolism of cysteine in hepatocytes isolated from Cdo1-null and wild-type mice. Hepatocytes from Cdo1-null mice produced more H2S and thiosulfate than did hepatocytes from wild-type mice. The greater flux of cysteine through the cysteine desulfhydration reactions catalyzed by CTH and CBS in hepatocytes from Cdo1-null mice appeared to be the consequence of their higher cysteine levels, which were due to the lack of CDO and hence lack of catabolism of cysteine by the cysteinesulfinate-dependent pathways. Both CBS and CTH appeared to contribute substantially to cysteine desulfhydration, with estimates of 56 % by CBS and 44 % by CTH in hepatocytes from wild-type mice, and 63 % by CBS and 37 % by CTH in hepatocytes from Cdo1-null mice.
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