期刊
BIOMATERIALS
卷 28, 期 32, 页码 4826-4835出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2007.07.039
关键词
biocompatibility; cell encapsulation; elastomer; nerve; scaffold; stem cell
资金
- NHLBI NIH HHS [R01 HL060435-05S1] Funding Source: Medline
- NIDCR NIH HHS [R01-DE13023, R01 DE013023-08, R01 DE016516-03] Funding Source: Medline
Encapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolcosebacate)-acrylate (PGSA). The scaffold porosity, swelling, mass loss, toxicity and mechanical properties, suggest that porous PGSA could be used to support the growth and differentiation of encapsulated cells. Neuroblastoma (NB) and human embryonic stem cells (hESCs) were encapsulated into the matrix and found to adhere to the material and interact with each other within 24h. After 7 days, encapsulated NB cells were found to grow, and form matrix fibrils and tissue. Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold. In vivo experiments showed that both porous scaffolds have similar biocompatibility profiles as non-porous PGSA, but porous PGSA promotes tissue ingrowth, as compared to non-porous PGSA. We therefore propose that porous PGSA scaffolds can provide a logistical template for 3D growth of cells and tissue engineering. (c) 2007 Elsevier Ltd. All rights reserved.
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