期刊
AMINO ACIDS
卷 45, 期 2, 页码 205-218出版社
SPRINGER WIEN
DOI: 10.1007/s00726-013-1494-0
关键词
FFPE tissue; Proteomics; Mass spectrometry; Modifications; Crosslinked proteins
资金
- Flemish Institute for Technological Research (VITO)
- Industrial Research Fund (KU Leuven)
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT)
Formalin-fixed paraffin-embedded (FFPE) tissues are a real treasure for retrospective analysis considering the amount of samples present in hospital archives, combined with pathological, clinical, and outcome information available for every sample. Although unlocking the proteome of these tissues is still a challenge, new approaches are being developed. In this review, we summarize the different mass spectrometry platforms that are used in human clinical studies to unravel the FFPE proteome. The different ways of extracting crosslinked proteins and the analytical strategies are pointed out. Also, the pitfalls and challenges concerning the quality of FFPE proteomic approaches are depicted. We also evaluated the potential of these analytical methods for future clinical FFPE proteomics applications.
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