期刊
PROTEOMICS
卷 7, 期 22, 页码 4032-4035出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200700491
关键词
bacillus subtilis; crosslinking; protein-protein interaction; SPINE; strep-tag
The detection and analysis of protein-protein interactions is one of the central tasks of proteomics in the postgenomic era. For this purpose, we present a procedure, the Strep-protein interaction experiment (SPINE) that combines the advantages of the Strep-tag protein purification system with those of reversible in vivo protein crosslinking by formaldehyde. Using two Bacillus subtilis regulator proteins, we demonstrate that this method is well suited to isolate protein complexes with high purity and virtually no background. Plasmids allowing the high-level expression of proteins carrying an N- or C-terminal Strep-tag in B. subtilis were constructed.
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