期刊
AMINO ACIDS
卷 39, 期 3, 页码 831-839出版社
SPRINGER WIEN
DOI: 10.1007/s00726-010-0538-y
关键词
L-Arginine; N-carbamylglutamate; Growth performance; Diarrhea; Intestinal morphology; Weaned pigs
资金
- Chinese Academy of Sciences [Kscx2-Yw-N-051]
- National 863 Program of China [2008AA10Z316]
- State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-TS-200817]
- Nanchang University, National Basic Research Program of China [2009CB118806]
- NSFC [30901040, 30901041, 30928018, 30828025, 30771558]
- National Fund of Agricultural Science and Technology [2006GB24910468]
- National Scientific and Technological [2006BAD12B02-5-2]
- USDA National Institute of Food and Agriculture [2008-35206-18764]
- Texas AgriLife Research [H-8200]
This study determined effects of dietary supplementation with l-arginine (Arg) or N-carbamylglutamate (NCG) on intestinal health and growth in early-weaned pigs. Eighty-four Landrace x Yorkshire pigs (average body weight of 5.56 +/- A 0.07 kg; weaned at 21 days of age) were fed for 7 days one of the three isonitrogenous diets: (1) a corn- and soybean meal-based diet (CSM), (2) CSM + 0.08% NCG (0.08%), and (3) CSM + 0.6% Arg. There were four pens of pigs per diet (7 pigs/pen). At the end of a 7-day feeding period, six piglets were randomly selected from each treatment for tissue collections. Compared with the control group, Arg or NCG supplementation increased (P < 0.05): (1) Arg concentrations in plasma, (2) small-intestinal growth, (3) villus height in duodenum, jejunum and ileum, (4) crypt depth in jejunum and ileum, (5) goblet cell counts in intestinal mucosae, and (6) whole-body weight gain in pigs. Real-time polymerase chain reaction and western blotting analyses revealed that both mRNA and protein levels for heat shock protein-70 (HSP70) were higher (P < 0.05) in the intestinal mucosae of Arg- or NCG-supplemented pigs than in the control group. Furthermore, the incidence of diarrhea in the NCG group was 18% lower (P < 0.01) than that in the control group. Collectively, these results indicate that dietary supplementation with 0.6% Arg or 0.08% NCG enhances intestinal HSP70 gene expression, intestinal growth and integrity, and the availability of dietary nutrients for whole-body weight gain in postweaning pigs fed a CSM-based diet. Thus, Arg or NCG is a functional ingredient in the weaning diet to improve nutrition, health, and growth performance of these neonates.
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