期刊
AMINO ACIDS
卷 39, 期 5, 页码 1493-1505出版社
SPRINGER WIEN
DOI: 10.1007/s00726-010-0618-z
关键词
Multidrug resistant; Antimicrobial peptides; Anti-inflammatory; Surface plasmon resonance; Cyclooxygenase; Histopathology
资金
- Indian Council Medical Research
- Department of Science and Technology
- Council of Scientific and Industrial Research
- Government of India
- SAIF facility, All India Institute of Medical Sciences
Anti-bacterial drug resistance is one of the most critical concerns among the scientist worldwide. The novel antimicrobial decapeptide SD-8 is designed and its minimal inhibitory concentration and therapeutic index (TI) was found in the range of 1-8 mu g/ml and 45-360, respectively, against major group of Gram positive pathogens (GPP). The peptide was also found to be least hemolytic at a concentration of 180 mu g/ml, i.e., nearly 77 times higher than its average effective concentration. The kinetics assay showed that the killing time is 120 min for methicillin-sensitive Staphylococcus aureus (MSSA) and 90 min for methicillin-resistant S. aureus (MRSA). Membrane permeabilization is the cause of peptide antimicrobial activity as shown by the transmission electron microscopy studies. The peptide showed the anti-inflammatory property by inhibiting COX-2 with a K (D) and K (i) values of 2.36 x 10(-9) and 4.8 x 10(-8) M, respectively. The peptide was also found to be effective in vivo as derived from histopathological observations in a Staphylococcal skin infection rat model with MRSA as causative organism.
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