Pulsed UV-light inactivation of poliovirus and adenovirus

Aims: To study the pulsed ultraviolet (UV) inactivation of poliovirus and adenovirus. Methods and Results: Viral suspensions of 2 ml volume were exposed to varying numbers of polychromatic light pulses emitted from a xenon flashlamp. Ten pulses produced an approximately 4 log(10) reduction in poliovirus titre, and no infectious poliovirus remained after 25 pulses. With adenovirus, 10 pulses resulted in an approximately 1 log10 reduction in infectivity. Adenovirus required 100 pulses to produce an approximately 3 log10 reduction in infectivity, and 200 pulses to produce a greater than 4 log10 reduction. Conclusions: Adenovirus was more resistant to pulsed UV treatment than poliovirus although both viruses showed susceptibility to the treatment. Significance and Impact of the Study: Pulsed UV-light treatment proved successful in the inactivation of poliovirus and adenovirus, and represents an alternative to continuous-wave UV treatment.