期刊
LUMINESCENCE
卷 22, 期 6, 页码 588-593出版社
WILEY
DOI: 10.1002/bio.1006
关键词
capillary electrophoresis; electrogenerated chemiluminescence; Ru(bpy)(3)(2+); trimethylamine; solid phase extraction
A capillary electrophoresis with electrogenerated chemiluminescence (CE-ECL) method for the determination of trimethylamine (TMA) in fish was studied. In the presence of TMA, ECL from the reaction of analyte and in situ generated tris(2,2'-bipyridyl)ruthenium(III) [Ru(bpy)(3)(3+)] at electrode surface could be produced. The ECL detection was performed using a Pt working electrode biased at 1.23 V (vs. Ag/AgCl) potential in a 10 mmol/L sodium borate buffer solution, pH 9.2, containing 3 mmol/L Ru(bPY)(3)(2+). A linear calibration curve (correlation coefficient = 0.9996) was obtained in the range 8 x 10(-5)-4 x 10(-8) mol/L for TMA concentration. Recoveries obtained were in the range 98.78-101.46%. The method was successfully applied for the assay of TMA in fish, in combination with solid phase extraction (SPE) disks for sample clean-up and enrichment. Copyright (c) 2007 John Wiley & Sons, Ltd.
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