期刊
MAGNETIC RESONANCE IN MEDICINE
卷 58, 期 5, 页码 871-879出版社
WILEY
DOI: 10.1002/mrm.21307
关键词
MRI; contrast agent; viral capsids; cowpea chlorotic mottle virus; gadolinium
资金
- NIBIB NIH HHS [R21EB005364, R01 EB00432] Funding Source: Medline
Viral capsids have the potential for combined cell/tissue targeting, drug delivery, and imaging. Described here is the development of a viral capsid as an efficient and potentially relevant MRI contrast agent. Two approaches are outlined to fuse high affinity Gd3+ chelating moieties to the surface of the cowpea chlorotic mottle virus (CCMV) capsid. In the first approach, a metal binding peptide has been genetically engineered into the subunit of CCMV. In a second approach gadolinium-tetraazacyclododecane tetraacetic acid (GdDOTA) was attached to CCMV by reactions with endogenous lysine residues on the surface of the viral capsid. T-1 and T-2 ionic relaxivity rates for the genetic fusion particle were R1 = 210 and R2 = 402 mM(-1)s(-1) (R2 at 56 MHz) and for CCMV functionalized with GdDOTA were R1 = 46 and R2 = 142 mM(-1)s(-1) at 61 MHz. The relaxivities per intact capsid for the genetic fusion were RI = 36,120 and R2 = 69,144 mM(-1)s(-1) (R2 at 56 MHz) and for the GdDOTA CCMV construct were R1 = 2,806 and R2 = 8,662 mM(-1)s(-1) at 61 MHz. The combination of high relaxivity, stable Gd3+ binding, and large Gd3+ payloads indicates the potential of viral capsids as high-performance contrast agents.
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