期刊
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
卷 293, 期 5, 页码 G963-G971出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpgi.00146.2007
关键词
incretin; gastrointestinal hormone secretion; enteroendocrine cells; intestinal lymph
资金
- NIDDK NIH HHS [DK56863, DK56910, DK76928, DK56930] Funding Source: Medline
- NIEHS NIH HHS [ES14464] Funding Source: Medline
Glucagon-like peptide-1 (GLP-1) is an important incretin produced in the L cells of the intestine. It is essential in the regulation of insulin secretion and glucose homeostasis. Systemic GLP- 1 concentrations are typically low in rodents, so it can be difficult to assay physiological levels or detect changes in response to nutrients. We have established a method of assaying GLP- 1 in response to nutrients using the intestinal lymph fistula model. Intraduodenal infusion of Intralipid ( 4.43 kcal/ 3 ml) induced a significant increase of lymphatic GLP- 1 concentration compared with saline control at the peak of 30 min. ( P < 0.001). Isocaloric and isovolumetric treatment with dextrin, a glucose polymer, also caused a significant fourfold increase in peak concentration at 60 min ( P = 0.001). These findings indicate that intestinal lymph contains high concentrations of postprandial GLP-1. Second, they reveal that GLP- 1 secretion into lymph occurs in response to both enteral carbohydrate and fat, but the response to dextrin occurs later than to Intralipid with peak times at 60 and 30 min, respectively. Third, the combination of Intralipid plus dextrin demonstrated an additive effect in the stimulation of GLP- 1 with peak at 30 min. These results indicate that assessment of levels in lymph is a novel and powerful means of studying the secretion of GLP- 1 and potentially other gastrointestinal hormones in vivo. Furthermore, the lymph fistula rat model provides insight into the gut hormone concentrations to which the neurons and cells in the lamina propria of the gut are likely exposed.
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