期刊
AMERICAN JOURNAL OF SURGERY
卷 194, 期 5, 页码 652-658出版社
EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC
DOI: 10.1016/j.amjsurg.2007.07.014
关键词
acinar cell; acute pancreatitis; bile and pancreatic juice; morphololgy; pancreatic clum rat
类别
资金
- NIDDK NIH HHS [K08 DK062805-05, K08-DK062805, K08 DK062805] Funding Source: Medline
Background: Bile-pancreatic duct ligation in rats causes acute pancreatic inflammation. We performed serial morphologic evaluation of the exocrine pancreas after duct ligation to facilitate further investigations using the model. Methods: The pancreas was excised from 74 rats after 0, 1, 3, 5, 24 or 48 hours of duct ligation or sham surgery. A pathologist evaluated I hematoxylin- and eosin-stained slide from each rat. Confirmatory immunostaining was performed with markers for apoptosis (activated caspase-3), proliferation (cyclin D3), neutrophils (myeloperoxidase), and macrophages (CD68). Results: Interstitial edema and white blood cell infiltration were apparent at 24 hours and increased at 48 hours. Progressive periods of duct ligation were characterized by ductular ectasia (I to 3 hours), acinar vacuolization (5 to 48 hours), leukocytic margination and neutrophil exocytosis (5 to 48 hours), ductule epithelium hypertrophy and proliferation (24 to 48 hours), and discernible loss of zymogen granules (48 hours). Conclusions: Ligation-induced acute pancreatitis in rats is a useful model to investigate early events in disease pathogenesis. (C) 2007 Excerpta Medica Inc. All rights reserved.
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