期刊
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
卷 43, 期 6, 页码 650-654出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S0003683807060142
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Galactomannan from seeds of Cyamopsis tetragonoloba (L.) Taub. (guar) was depolymerized using immobilized enzymatic preparation celloviridin. A set of fragments whose molecular weights varied from 12.6 to 245.6 kDa was obtained. Sulfated derivatives of components of all fractions were synthesized, in which the content of HSO3- groups was 48.05 +/- 2.31%. All preparations exhibited anticoagulant activity, which was recorded in vitro in two tests - aIIa and aXa. The antithrombin activity (aIIa) was high (up to 65-87 U/mg) and did not depend on the molecular weight of a sulfated derivative; in the second test (aXa), the effect of molecular weight was observed. Biospecific electrophoresis allowed us to detect the ability of galactomannan sulfates to form complexes with protomine sulfate, a classic antidote to heparin.
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