期刊
BIOELECTROCHEMISTRY
卷 71, 期 2, 页码 164-171出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2007.04.001
关键词
electroporation; dielectrophoresis; cell separation; membrane permittivity
Electroporation is a phenomenon during which exposure of a cell to high voltage electric pulses results in a significant increase in its membrane permeability. Aside from the fact that after the electroporation the cell membrane becomes more permeable, the cells' geometrical and electrical properties change considerably. These changes enable use of the force on dielectric particles exposed to non-uniform electric field (dielectrophoresis) for separation of non-electroporated and electroporated cells. This paper reports the results of an attempt to separate non-electroponated and electroporated cells by means of dielectrophoresis. In several experiments we managed to separate the non-electroporated and electroporated cells suspended in a medium with conductivity 0.174 S/m by exposing them to a non-uniform electric field at a frequency of 2 MHz. The behaviour of electroporated cells exposed to dielectrophoresis raises the presumption that in addition to conductivity, considerable changes in membrane permittivity occur after the electroporation. (c) 2007 Elsevier B.V. All rights reserved.
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