期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 70, 期 11, 页码 969-974出版社
WILEY
DOI: 10.1002/jemt.20502
关键词
imaging MS; TOF-SIMS; 3D imaging; thyroid tumor cells; cell membrane; phospholipids; potassium compartment
The distribution of phosphocholine ions (m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF-SIMS. Repeated sputtering with a C-60(+) source and subsequent analysis with a Bi-3(+) gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane (m/z 184) and intracellular membranes (m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane-encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells.
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