期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 39, 期 11, 页码 2905-2914出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2007.06.017
关键词
phosphate solubilization; phosphate-solubilizing bacteria; iron-rich acidic soils; GDH
类别
With the aim to explore the possible role of mineral phosphate-solubilizing bacteria (PSB) in phosphorus (P) cycling in iron-rich, acidic soils, we conducted a survey of PSB naturally colonizing a limonitic crust in the south-east region of Venezuela (Bolivar State). A total of 130 heterotrophic bacterial isolates showing different degrees of mineral tri-calcium phosphate (Ca(POM4)(2))-solubilizing activities were isolated from NBRIP plates. In contrast, no isolates showing iron phosphate (FePO4)- or aluminum phosphate (AlPO4)-solubilizing activities were detected by this experimental approach. The 10 best Ca-3(PO4)(2)-SOlubilizers were selected for further characterization. These isolates were shown to belong to the genera Burkholderia, Serratia, Ralstonia and Pantoea by partial sequencing analysis of their respective 16S rRNA genes. All the PSB isolates were able to mediate almost complete solubilization of Ca-3(PO4)(2) in liquid cultures; in contrast, the PSB isolates were less effective when solubilizing FePO4. Two groups of PSB isolates were clearly differentiated on the basis of their Ca-3(PO4)(2) solubilization kinetics. Acidification of culture supernatants seemed to be the main mechanism for P solubilization. Indeed, gluconic acid was shown to be present in the supernatant of five isolates. Furthermore, detection of genes involved in the production of this organic acid was possible in three isolates by means of a PCR protocol. (c) 2007 Elsevier Ltd. All rights reserved.
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