期刊
CELL BIOLOGY INTERNATIONAL
卷 31, 期 11, 页码 1316-1322出版社
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cellbi.2007.05.003
关键词
2,4-D; 2,4-D DMA; human lymphocytes; sister chromatid exchanges; cell-cycle progression; mitotic activity
类别
The genotoxic effects of 2,4-D and its commercial derivative 2,4-D DMA were studied by measuring sister chromatid exchange (SCE), cell-cycle progression and mitotic index in human whole blood (WBC) and plasma leukocyte cultures (PLC). Concentrations of 10, 25, 50 and 100 mu g herbicide/ml were used during 72 h. In WBC, a significant increase in SCE frequency was observed within the 10-50 mu g 2,4-D/ml and 25-100 mu g 2,4-D DMA/ml dose range. Contrarily, in PLC, none of the concentrations employed affected the SCEs frequency. A significant delay in cell proliferation was observed in WBC after treatments with 25 and 50 mu g 2,4-D/ml and 50 and 100 mu g 2,4-D DMA/ml. In PLC, only 100.0 mu g 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMA were more potent genotoxic agents in the presence of human red cells. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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